University Sophisticated Instrumentation Centre

Scanning Electron Microscope (SEM):

A Scanning Electron Microscope (JSM 6490) scans is a focused electron beam over a surface to create an image. The electrons in the beam interact with the sample and produce various signals that is used to obtain information about the surface topography and composition. It can be use both under low & high vacuums that depending on the nature of the specimens. Low vacuum with low kV (energy) provides the user the longer duration for scanning the materials, organic specimens, before it is charged. The instrument has been coupled with an optional accessory, viz., EDS 133, EV Dry Detector (INCA x-act) of OXFORD instruments, UK, which has enhanced the application range in a manner that any metal associated with the biological material or in isolation or in the form of alloy may be identified and also quantified. The sample preparation for SEM facility has also been supported with the two important units, viz., Sputter Coater of JEOL, Japan (JFC 1600, Auto Fine Coater) and Critical Point Dryer (CPD) Emitech K 850 of Quorum Technology of UK.

Fourier Transform Infra-Red Spectrometer (FTIR):

Fourier Transform-Infrared Spectroscopy (FTIR) is an analytical technique used to obtain an infrared spectrum of absorption or emission of organic (and in some cases inorganic) materials. The FTIR has been supplemented with an accessory called Attenuated Total Reflectance (ATR) to enhance the use and application of the instrument in way that with this facility any form of material, i.e., filmy/pellet; liquid, including liquids of flammable nature may be studied; rather that its limited use of getting spectra of the solids on the powdered form through making pellet by mixing the same with the KBr under a hydraulic press

Fast Performance Liquid Chromatograph (FPLC):

Fast Performance Liquid Chromatograph (FPLC) is a form of medium pressure chromatography that is used to analyze or purify mixture of proteins and other biomolecules. The FPLC (main AKTA 10) has been provided with other accessories which include Sephadex G75, Sephacry1 S – 200, DeaeSepharose Fast FL, CM Sepharose fast flow short & long column holders, AKTA user kit etc. to make the facility of broader usage

X-ray diffraction (XRD):

X-ray Diffraction (XRD) is a powerful technique for characterizing crystalline and polycrystalline materials. It provides information of structures, phases, texture, and other structural parameters, such as average grain size, crystallinity, strain, and crystal defects. The system is equipped with Goniometer, X-ray source, Tube housing, Mount, Sample holder and Detector with Optics and Slit systems. The system has DIFFRAC (EVA evaluation software), DIFFRAC (TOPAS software) for diffractogram analysis and ICDD PDF-4 Axiom 2020 database.

Inverted Laser Scanning Confocal Microscope (ILSCM):

Inverted Laser Scanning Confocal Microscope is a fluorescence-imaging technique that produces exquisitely sharp optical sections through biological specimens by exciting a laser over the sample and collecting emission data, point by point, and reconstructed with a computer, allowing threedimensionalreconstructions of topologically complex objects.The ILSCM (ZEISS, LSM 900) equipped with multichannel fluorescence imaging Z-stack, colocalzation, time-lapse imaging. FRET, FRAP, Photo activation and conversion. It is capable of highly sensitive spectral confocal imaging of fixed as well as live biological samples.

Preparative HPLC System:

Prep HPLC is used to separate and refine high-purity target compounds from a mixed solution. It is used to fraction high-purity compounds required for subsequent evaluation, analysis, and processes in the shortest possible time. The Preparative HPLC (SHIMADZU, Model: LC-20 AP) equipped with SPD-M 40A Photodiode Array Detector which wavelength rage is from 190-800nm, CBM 20A system controller, Fraction collector FRC-10A, C18 & C8 column and LabSol ADD PDA connect CS ready software